SCHEDULE 13D/A

Actuate Therapeutics Inc

Common Stock, par value $0.0001 per share · CUSIP: 005083100

Event date: 2025-05-22

Reporting persons may disclose overlapping interests. Their amounts are shown individually and are not added into a filing total.

Filing-level shares

Unavailable

Percent of class

Unavailable

• BIOS FUND I, LPReported CIK 0001697316

  Shares: 525,797.00 · Class: 2.7%

• BIOS FUND I QP, LPReported CIK 0001700297

  Shares: 307,538.00 · Class: 1.6%

• BIOS FUND II, LPReported CIK 0001714576

  Shares: 308,211.00 · Class: 1.6%

• BIOS FUND II QP, LPReported CIK 0001716869

  Shares: 1,006,788.00 · Class: 5.1%

• BIOS FUND II NT, LPReported CIK 0001728851

  Shares: 134,776.00 · Class: 0.7%

• BIOS FUND III, LPReported CIK 0001791917

  Shares: 383,791.00 · Class: 2.0%

• BIOS FUND III QP, LPReported CIK 0001791916

  Shares: 2,506,667.00 · Class: 12.8%

• BIOS FUND III NT, LPReported CIK 0001791910

  Shares: 404,814.00 · Class: 2.1%

• BP DIRECTORS, LPReported CIK 0001813311

  Shares: 116,801.00 · Class: 0.6%

• BIOS ACTUATE CO-INVEST I, LPReported CIK 0001771378

  Shares: 300,749.00 · Class: 1.5%

• BIOS ACTUATE CO-INVEST II, LPReported CIK 0001825398

  Shares: 2,094,650.00 · Class: 10.7%

• BIOS ACTUATE CO-INVEST III, LPReported CIK 0001956704

  Shares: 573,394.00 · Class: 2.9%

• BIOS CLINICAL OPPORTUNITY FUND, LPReported CIK 0001988639

  Shares: 1,259,427.00 · Class: 6.4%

• BIOS 2024 CO-INVEST, LPReported CIK 0002028708

  Shares: 125,000.00 · Class: 0.6%

• BIOS EQUITY PARTNERS, LPReported CIK 0001813314

  Shares: 950,136.00 · Class: 4.8%

• BIOS EQUITY PARTNERS II, LPReported CIK 0001813313

  Shares: 1,750,524.00 · Class: 8.9%

• BIOS EQUITY PARTNERS III, LPReported CIK 0001831976

  Shares: 5,963,316.00 · Class: 30.4%

• BIOS EQUITY COF, LPReported CIK 0002000747

  Shares: 1,384,427.00 · Class: 7.1%

• CAVU MANAGEMENT, LPReported CIK 0001813270

  Shares: 8,663,976.00 · Class: 44.0%

• BIOS CAPITAL MANAGEMENT, LPReported CIK 0001813845

  Shares: 10,048,403.00 · Class: 51.0%

• CAVU ADVISORS, LLCReported CIK 0001813310

  Shares: 8,663,976.00 · Class: 44.0%

• BIOS ADVISORS GP, LLCReported CIK 0001813844

  Shares: 10,048,403.00 · Class: 52.4%

• LESLIE W. KREISReported CIK 0001813316

  Shares: 8,733,351.00 · Class: 44.4%

• AARON G.L. FLETCHERReported CIK 0001789490

  Shares: 10,109,376.00 · Class: 51.3%

Amounts are as disclosed in this filing. Schedule type alone does not establish investment intent.