SCHEDULE 13D/A

Tenaya Therapeutics Inc

Common Stock, par value $0.0001 per share · CUSIP: 87990A106

Event date: 2025-05-07

Reporting persons may disclose overlapping interests. Their amounts are shown individually and are not added into a filing total.

Filing-level shares

Unavailable

Percent of class

Unavailable

• The Column Group III, LPReported CIK 0001668159

  Shares: 4,414,720 · Class: 2.7%

• The Column Group III-A, LPReported CIK 0001668085

  Shares: 4,985,570 · Class: 3.1%

• The Column Group III GP, LPReported CIK 0001790965

  Shares: 9,400,290 · Class: 5.8%

• The Column Group Opportunity III, LPReported CIK 0001931825

  Shares: 49,313,559 · Class: 30.3%

• The Column Group Opportunity III GP, LPReported CIK 0001932874

  Shares: 49,313,559 · Class: 30.3%

• TCG Opportunity III GP, LLCReported CIK 0001932445

  Shares: 49,313,559 · Class: 30.3%

Amounts are as disclosed in this filing. Schedule type alone does not establish investment intent.